regarding volume to be maintained in 12ml ultracentrifuge tubes during ultracentrifugation (divya teja)

Nikola Wenta via methods%40net.bio.net (by Nikola.Wenta from nottingham.ac.uk)
Mon May 21 15:38:59 EST 2012


Hi Divya,
why don't you simply split your 12 ml into two times 6 ml? You would need a counterbalance for the centrifuge anyway.
Cheers,
Niko

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>    1. regarding volume to be maintained in 12ml ultracentrifuge
>       tubes during ultracentrifugation (divya teja)
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> Message: 1
> Date: Mon, 21 May 2012 15:22:26 +0200
> From: divya teja <divya.medbio from gmail.com>
> Subject: regarding volume to be maintained in 12ml ultracentrifuge
> 	tubes during ultracentrifugation
> To: methods from magpie.bio.indiana.edu
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> 
> hello everyone
> 
>  I wanted to isolated rafts from the mouse neuronal cell 
> lines using ultracentrifuge.
> So, the am having 12ml centrifuge tubes that to be used for 
> ultracentrifugation, but my sample volume itself is 12ml exactly.
> 
> So, my doubts are:
>    1.  can i use 12ml centrifuge tubes for my 12ml sample?????
>    2.  will there be any spillage of sample????????
>    3.  Actually what are the minimum and maximum volumes to 
> be maintained in 12ml ultra centrifuge tubes?????????
> 
> 
>  Can you please help me, in clearing my doubts?.
> 
> 
>    thank you
> 
> 
> 
>    regards
>    divya
> 
> 
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