cloning oligos

Ed Siefker via methods%40net.bio.net (by ebs15242 from gmail.com)
Thu May 24 10:48:33 EST 2012


I'm trying to clone a short sequence by annealing two complimentary
oligos with incompatible overhangs.  Am I correct in understanding
that if I'm doing directional cloning and don't dephosphorylate my
vector, I do not need to phosphorylate the oligos?



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