Quick question about Quikchange mutagenesis (substitiution)

Phelan, Paul J. via methods%40net.bio.net (by Paul.Phelan from tufts.edu)
Mon Jul 8 13:44:12 EST 2013


I have a quick question about large substitutions using the Stratagene/Agilent Quikchange kit.  Does anyone know if Quikchange can be used to make substitutions of 5 to 6 bases at one site?  I think it probably can, but I can't find a straight answer easily on-line to confirm it.

Thanks in advance,

Paul Phelan
Tufts University Boston



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