Alternatives to agar

Paul A. Vescio vescip at rpi.edu
Wed Dec 7 03:04:08 EST 1994


In Article <D0EE07.LHr at info.bris.ac.uk>, glsjb at mail.bris.ac.uk (S. J. Bale)
wrote:
>
>Does anyone out there know anythign about pluronic polyol (ppol) as a means of
>solidifying media (instead of agar).
>
>I need someting which will be liquid at or near room temperature, and I
>seem to recall PPol as fitting the bill.
>
Gelatin would work well as it liquifies at 25C.  Only drawback would be if
the organism(s) you are working with contain the enzyme gelatinase.  You can
check this by making tubes of media with gelatin and incubating them at
whatever temp. you need. To test for liquification just put the tube on ice
for 15 minutes or so and see if the gelatin solidifies or not.


Paul A. Vescio
Biology Dept.
Rensselaer Polytechnic Institute

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