Expression of cloned archeal genes

Dan Diaz bl275 at cleveland.Freenet.Edu
Thu Dec 15 20:12:11 EST 1994



im posting this for a friend (really).  he is attempting to express an
archeal gene in e. coli without much luck.  we took a peek-see at the codon
usage and found (yikes) that 20% of his codons are used rarely in e. coli.

he is now thinking of using a eukaryotic expression system and is concerned
about glycosylation by, e.g., Pichia.

what are your suggestions for expression of archeal genes?



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