transducing recA w/o tetR ?
Alberto I Roca
roca at macc.wisc.edu
Tue Jul 5 14:02:00 EST 1994
In article <CsH3DG.76H at usenet.ucs.indiana.edu> the End,
jgraham at bronze.ucs.indiana.edu writes:
>Has anyone used P1vir to transduce E. coli to recA- using a marker other
>tn10(tetR) ? My host strain is already tetR and I have been trying to
an alternative strategy is to remove the tetR from your starter strain.
protocol (1) selects for tet sensitive strains therefore losing the tetR
(transposon pops out). then you can perform P1 transduction w/ recA-tetR
(1) Davis, R. W., Botstein, D., and Roth, J. R., Advanced Bacterial
Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1980,
p90 & 205.
Alberto I. Roca Internet: roca at macc.wisc.edu
Biochemistry Bitnet: roca at wiscmacc
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