transducing recA w/o tetR ?

the End jgraham at bronze.ucs.indiana.edu
Tue Jul 5 10:08:03 EST 1994


Hello,

Has anyone used P1vir to transduce E. coli to recA- using a marker other than
tn10(tetR) ? My host strain is already tetR and I have been trying to move 
a tn9-200 linked recA marker from DB1319 (recA938::Tn9-200). So far no 
luck. 

Does anyone see any inherent problems in this strategy (eg. transducing a 
tn9 linked mutation) ?

thanks,

Jim
J. E. Graham 




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