transducing recA w/o tetR ?

the End jgraham at bronze.ucs.indiana.edu
Tue Jul 5 17:58:15 EST 1994


Patricia,

Thanks for the tip. I can't find any references to a requirement for 
homologous recombination in P1 transduction. Any theories on this ? 
Unfortunately my source of recA:Tn9 mutation is a RecD- mutant as well.

I'm titering this lysate more carefully at this point. I think I'll wait
on the "chlortetracycline".

Jim
J. E. Graham 




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