transducing recA w/o tetR ?
mbrgw at s-crim1.dl.ac.uk
Wed Jul 6 05:46:13 EST 1994
In article <2vccc4$5mj at news.bu.edu> pfoster at bu.edu (Patricia Foster) writes:
> Hands up everyone who has tried but failed to get the
>Bochner method to work.
Why use that? - the Fusaric Acid method according to Maloy and Nunn (1981),
J. Bacteriol. 145, 1110-1112, works very well. This is a modification of
another method (Bochner? I don't remember) which was designed for
Salmonella typhimurium. Roughly 80-90% of colonies on these plates were
(in my hands) Tet sensitive.
Robin Walters. Robert Hill Institute, Sheffield UK.
A fact is an opinion that everyone agrees with.
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