transducing recA w/o tetR ?

R.G. Walters mbrgw at
Wed Jul 6 05:46:13 EST 1994

In article <2vccc4$5mj at> pfoster at (Patricia Foster) writes:
>  Hands up everyone who has tried but failed to get the
>Bochner method to work.

Why use that? - the Fusaric Acid method according to Maloy and Nunn (1981), 
J. Bacteriol. 145, 1110-1112, works very well.  This is a modification of
another method (Bochner? I don't remember) which was designed for 
Salmonella typhimurium.  Roughly 80-90% of colonies on these plates were
(in my hands) Tet sensitive. 

Robin Walters.                      Robert Hill Institute, Sheffield UK.

A fact is an opinion that everyone agrees with.

More information about the Microbio mailing list