transducing recA w/o tetR ?
mbrgw at s-crim1.dl.ac.uk
Wed Jul 6 11:00:20 EST 1994
[On fusaric acid selection...]
>: Roughly 80-90% of colonies on these plates were (in my hands) Tet sensitive.
One thing I found helped was incubating at 30C. The selection seems more
effective if the cells are growing/dividing more slowly.
Oh - and of course you have to grow the cells in the absence of Tet prior
to plating them out. Else all the ones you want are dead :-(
Robin Walters. Robert Hill Institute, Sheffield UK.
A fact is an opinion that everyone agrees with.
More information about the Microbio