Control of bacterial lag phase

Mr V Mulholland lsrdp at csv.warwick.ac.uk
Mon Jul 11 05:30:23 EST 1994


In article <1994Jun29.214012.23491 at ucl.ac.uk>, dmcbbap at ucl.ac.uk (dmcbbap)
wrote:

: > Dear net people,
: >                I have been working on lag phase of bacterial growth.
: > What i need is a compound or treatment that leads to extension of the
: > lag phase but does NOT affect growth rate.......(rest deleted)

One way to increase lag is to subculture from a culture kept at stationary
phase. This is outlined in "Growth of the Bacterial Cell" (J.L. Ingraham,
O. Maaloe & F.C. Neidhardt) Sinauer Associates, Inc. Sunderland, Ma. 
ISNB 0-87893-352-2. p 235

	"... if a culture of E. coli is grown to stationary phase in minimal-
salts medium with a low concentration of glucose (around 0.02%), it will
reinitiate growth (as measured by mass increase) with virtually no lag phase
when transferred to a fresh medium after having been held in the stationary 
phase for periods of up to several hours. If held for several days in the 
stationary phase, a lag time of several hours might result. On the other 
hand, if the glucose concentration were high (approximately 0.2%) in the
initial culture, a lag period would result after only a brief holding in the
stationary phase, presumably because of the higher accumulation of metabolic
by-products - principally acetate in the case of E. coli."

You could also extend lag if the culture is incubated at a suboptimal
temperature, but this would alter the growth rate.

Hope this helps,

Vince.
			  
			  
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