Variability in lac fusions assays

the End jgraham at bronze.ucs.indiana.edu
Mon Jul 18 12:03:52 EST 1994


I am seeing great variability in parallel identical E.coli cultures
which have full length lacZ fusions to a strong promoter (ParaBAD).
Anyone who has struggled with this please send me a note.

(eg. how many Miller units is "too many", preferred media, induction time, ect.)

Thanks,

Jim
J. E. Graham 





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