help with very large plasmids

M.A.Schmidt infekt at uni-muenster.de
Wed Nov 9 04:21:08 EST 1994


Hi out there,
we are seeking help with the isolation of very large plasmids (> 150 kb)
from E.coli. Though we have tried a number of different techniques we
could not manage a reliable isolation. Does anyone know or has experinece
with these kind of size problems? Any help will be greatly appreciated.
With many thanks
M.Alexander Schmidt



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