Taxonomy of Thermus sp.

Wiggy A.C.Hilton at
Wed Oct 5 11:58:59 EST 1994

In article <36ud2n$isf at>, Ronan.Okennedy at UCG.IE
(Ronan.Okennedy) wrote:

> >Hi Folks,
> >
> >A question for the taxonomists out there.
> >
> >I have approx 100 Thermus sp. isolates. I want to find if any could
> >potentially be new Thermus species ie are different from Thermus aquaticus
> >YT1, Thermus brockianus and Thermus thermophilus. I don't want to find
> >how related they are to one another just whether they are different
> >from valid species. There are a lot of Thermus isolates around but
> >taxonomy is kind of in its infancy in this species.
> >
> >Now I know I can do DNA/DNA hybridisation but that takes a lot of
> >time if you are not set up and are starting from scratch. I can do
> >16SrRNA RFLP patterns either on PCR'd 16S or by southerns with a 16s
> >probe. I know from a students work that Fusobacterium species have
> >unique rRNA RFLP patterns specific for the species but does this
> >apply to other organisms. Is there any other method that is quick
> >and reasonable valid. I don't want to have to have to sequence 100
> >16SrRNA variable regions! I don't want to propose anything as a new
> >species on whatever method I settle on, Ijust want pointers towards novel
> >species. Classical microbiology on 100 isolates will take too much
> >work at this stage.
> >
> >Many thanks
> >
> >Duncan
> >-----------------------------------------------------------------------------
> >Duncan Clark                        | Internet:    duncan at
> >G4ELJ                               | Compuserve:  100015.1406 at
> >-----------------------------------------------------------------------------
> What about M13 fingerprinting?..based on 2 clusters of 15bp sequence within
> protein gene III gene. Single starnded M13 DNA can be used as an RFLP probe
> for repeditive sequences in bacteria and eucaryotes. Has the advantage that
> you don't need to do any extensive probe preparation.
> refs
> Vassart et al(feb 1987) Science 235 pp 234-235
> Vassart (Apr1987) Trends in Genetics vol3 p89
> There are refs for applications in a procaryotes and eucaryotes which I
> have somewhere. If you are interested contact me at above mail address.
> Ronan O'Kennedy
> Ronan.Okennedy at
> Fermentation Technology
> Department of Microbiology
> University College
> Galway
> Ireland

You could also try RAPD which has been used with success as a typing method.


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