Increase toxin production of B. fragilis

robiso at vt.edu robiso at MAIL.VT.EDU
Mon Oct 17 20:41:45 EST 1994


I work with a strain of Bacteroides fragilis (gram neg, anaerobic rod) that
produces an enterotoxin.   Does anyone have any information that may help
me increase my toxin yields from a standard BHI broth medium ?  We have
tried metal ions, bile acids, elevated temperatures, pH variations,
harvesting (time) variations, and enzyme inhibitors, but none seem to
increase the toxin yeilds.  We
currently purify approximately 50 micrograms of toxin per liter of culture
filtrate.
PLEASE respond to my e - mail at:       robiso at mail.vt.edu

Richard J. Obiso, Jr.
Virginia Tech
Department of Biochemistry and Anaerobic Microbiology
1400 Prices Fork Road
Blacksburg, VA  24060 - 0305
(703) 231 - 5094
robiso at mail.vt.edu






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