Ion exchange chromatography/ Bio-Rad assay

Svein Horn horn at
Mon Oct 31 06:21:00 EST 1994


I have analyzed some protein samples from E. choli.
I used an anionic exchanger to separate the proteins ( used different NaCl -solut-
ions 0.1,0.2,0.5 and 1.0 M).
I also messured the protein consentration in each sample with Bio-Rad.

PROBLEM: The last sample( 1.0 M) gave a big top in the exchange experiment
         ( Means high cons. of amino acids), But Bio-Rad showed a very low 
          Protein cons.. I can't explain this. Anyone?

         ( IN the 3 other samples the two methods agreed)


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