Spontaneous Spectinomycin resistance ?
jgraham at bronze.ucs.indiana.edu
Thu Sep 1 10:47:32 EST 1994
I am seeing a relatively high rate of acquisition of spectinomycin
resistance in the E. coli strain I am working with (CSH20 from Miller's
old kit). It appears that all cells in a cuture are somewhat resistant,
and that 60 or so colonies will form from approximately 100 ul of
competent cells spread on a plate.
Anyone else see this ? Any relevant knowledge of mechanisms ?
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J. E. Graham
Biology and Chemistry Departments
Indiana University Bloomington
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