Spontaneous Spectinomycin resistance ?

[Jarmo Niemi]

the End (jgraham at bronze.ucs.indiana.edu) wrote:
> Hello,

> I am seeing a relatively high rate of acquisition of spectinomycin 
> resistance in the E. coli strain I am working with (CSH20 from Miller's
> old kit). It appears that all cells in a cuture are somewhat resistant,
> and that 60 or so colonies will form from approximately 100 ul of 
> competent cells spread on a plate.


> Anyone else see this ? Any relevant knowledge of mechanisms ?

> Thanks much,

> E-mail copies of all posts,

> Jim
> J. E. Graham 
> Biology and Chemistry Departments 
> Indiana University Bloomington 



--
Jarmo Niemi,Biochemistry,U.of Turku,jarnie at utu.fi,http://www.utu.fi/~jarnie/
"Rommel, you old fox, I_read_your_book_!"
				-Patton