gjs at aber.ac.uk
Tue Sep 6 03:27:38 EST 1994
In article <CvIHKG.Jp9 at info.bris.ac.uk> glsjb at mail.bris.ac.uk (S. J. Bale) writes:
>Kevin P. O'Connell (oconne18 at pilot.msu.edu) wrote:
>: In Article <77783025834n12 at 188.8.131.52>
>"Chris=Michiels%LMM%AGR at agr.kuleuven.ac.be" says:
>: > How (medium, OD,...) should one grow cultures for subsequent freezing or
>: > What is the best cryoprotectant? At what concentration?
>: > What about anaerobes?
>H. Hippe (1984). Maintenance of Methanogenic Bacteria In: Maintenance of
>Microorganisms, A Manual of Laboratory Methods (eds. B. E. Kisop Snell,
>J.J.) Academic Press, London,
>for anaerobes. We;ve used it for SRB and others successfully.
You should do a name search on Kirsop, she has been involved in culure
collections and cell preservation techniques for a long time. It should bring
you more than enough info to be getting on with.
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