Culture preservation

Gaz gjs at
Tue Sep 6 03:27:38 EST 1994

In article <CvIHKG.Jp9 at> glsjb at (S. J. Bale) writes:

>Kevin P. O'Connell (oconne18 at wrote:
>: In Article <77783025834n12 at>
>"Chris=Michiels%LMM%AGR at" says:
>: > How (medium, OD,...) should one grow cultures for subsequent freezing or
>: > What is the best cryoprotectant? At what concentration?
>: > What about anaerobes?


>H. Hippe (1984). Maintenance of Methanogenic Bacteria In: Maintenance of
>Microorganisms, A Manual of Laboratory Methods (eds. B. E. Kisop Snell,
>J.J.) Academic Press, London,

>for anaerobes. We;ve used it for SRB and others successfully.

You should do a name search on Kirsop, she has been involved in culure 
collections and cell preservation techniques for a long time. It should bring 
you more than enough info to be getting on with.


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