Culture preservation

[Gaz]

In article <CvIHKG.Jp9 at info.bris.ac.uk> glsjb at mail.bris.ac.uk (S. J. Bale) writes:


>Kevin P. O'Connell (oconne18 at pilot.msu.edu) wrote:
>: In Article <77783025834n12 at 134.58.40.4>
>"Chris=Michiels%LMM%AGR at agr.kuleuven.ac.be" says:
>: > How (medium, OD,...) should one grow cultures for subsequent freezing or
>lyophilisation?
>: > What is the best cryoprotectant? At what concentration?
>: > What about anaerobes?

>Try 

>H. Hippe (1984). Maintenance of Methanogenic Bacteria In: Maintenance of
>Microorganisms, A Manual of Laboratory Methods (eds. B. E. Kisop Snell,
>J.J.) Academic Press, London,


>for anaerobes. We;ve used it for SRB and others successfully.

You should do a name search on Kirsop, she has been involved in culure 
collections and cell preservation techniques for a long time. It should bring 
you more than enough info to be getting on with.

Gaz