Need help on growing B. stearothermophilus

Dan Diaz bl275 at cleveland.Freenet.Edu
Fri Sep 9 08:54:27 EST 1994


So like - i am studing this E. coli enzyme which appears to have a
counterpart (analogue) in B.s.  Ive tried growing the B.s. in LB at 55 'C -
sometimes the cultures grow OK - othertimes I observe 'floaties' which i
assume to be lysed cells.  Have I grown them too long?  Have they
sporulated?  Whats a good absorbance reading for a mid-log phase culture of
stearo?  Thanks mucho

diz



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