Bacteria in human nose

Candace Krepel ckrepel at post.its.mcw.edu
Thu Sep 15 12:56:48 EST 1994


: At 02:09 AM 9/15/94 GMT, Graham Higgins wrote:
: >
: > My friend is working on a project and would like to know what species of
: >bacteria would normally be found in the human nose.  Also, any information
: >on 'brain and heart infusion' media for growing bacteria (preparation,
: >whether it is selective for certain bacteria species, etc) would be
: >appreciated.  Please send me e-mail if you have any information.  Thanks.

If your friend wants to make his/her own media, that can be done with a
pressure cooker with a pressure indicator dial.  The powder should first be
completely dissolved with heat and stirring, then pressure-cooked
(autoclaved) for 15 minutes at 15 pounds of pressure.  (That is, start
timing after proper pressure has been reached).  Allow the media to cool
enough so that uou can touch the container to your cheek without burning
yourself - warmer will cause excess condensation, cooler will allow it to
set up in the container - then pour it into your sterile plates.
	Since your friend is apparently planning to grow some bacteria, it
would be a good idea for him/her to contact the microbiology lab at a local
hospital and ask them if they would be willing to accept the grown plates
for disposal, and a biohazard bag for that purpose.  The rest of us don't
want your friend throwing bacterial waste into the regular garbage <g>.  I
should think the lab would be happy to autoclave the plates.

Candy Krepel
Surgical Microbiology Research Lab, Medical College of Wisconsin



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