Stripping Biotin-labeled Membranes
PASSICK, JANELL ELIZABETH
jep5651 at zeus.tamu.edu
Thu Sep 15 18:01:00 EST 1994
Hi!
I was wondering if anyone out there has had any experience stripping
biotinylated probes off of nitrocellulose membranes (Gene Images) for
reprobing. I am currently using biotin-labeled pcr products to
hybridize to time-course RNA samples and I am having trouble stripping
any probes longer than 100 bp off of my membranes, although the kit
instructions say it should be possible. Any help would be greatly
appreciated. Thanks!
Janell E. Passick
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