Stripping Biotin-labeled Membranes

PASSICK, JANELL ELIZABETH jep5651 at zeus.tamu.edu
Thu Sep 15 18:01:00 EST 1994


Hi!

I was wondering if anyone out there has had any experience stripping 
biotinylated probes off of nitrocellulose membranes (Gene Images) for 
reprobing.  I am currently using biotin-labeled pcr products to 
hybridize to time-course RNA samples and I am having trouble stripping 
any probes longer than 100 bp off of my membranes, although the kit 
instructions say it should be possible.  Any help would be greatly 
appreciated.  Thanks!

Janell E. Passick




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