Help with S-layers
WINNINGHAM THOMAS AN
winningh at benji.Colorado.EDU
Tue Sep 20 00:05:14 EST 1994
I'm a physicist working with the S-layers of
Solfolobus Sulfataricus and Solfolobus Acidocaldarius.
I grow these bugs in my lab, but lately I've encountered
some problems that hopefully someone reading this board
will know how to remedy.
We isolate the whole sacs of S-layer and then sonicate them
to break them up into flat patches that we then deposit
onto a substrate. Lately, we have had problems with the patches
clumping together. Futhermore, we've been having trouble with
small contaminations sticking to the S-layer. These contaminations
are roughly spherical and about 10nm in diameter. These
contaminations cause problems because they obscure the holes of the S-layer.
If anyone has any ideas about how I can reduce the clumping and
the contamination, I would appreciate hearing from you.
University of Colorado, Boulder
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