agarase purification

Thu Sep 22 16:58:51 EST 1994

Hi!  I am interested in purifying an agarase produced by a marine
bacterium.  I was wondering if anyone has experience with affininity
chromatography to purify agarases.  Could you please tell me what type of
column (what you packed it with) and reagent you used to elute it with.  I'd
also be interested in any problems, if there were any while attempting to do

Thanks for any help you can give me.

Lynne Whitehead
Grad. student
U. of Maryland - Microbiology Dept.
College Park,MD

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