infected SEMEN, help needed!
bcapstone at aol.com
Mon Sep 26 21:20:08 EST 1994
In article <366hh3$10k at charm.magnus.acs.ohio-state.edu>,
dstothar at magnus.acs.ohio-state.edu (Diane Stothard) writes:
<<<<<<Get ahold of some prokaryotic 16S rRNA primers and so some PCR on
your sample. You will have to clone your products and sequence them
individually since it appears that you have several microbes. After that,
you can BLAST your sequence to GenBank and receive a list of the closest
relatives to your critters. If
you're lucky, you may not have uncovered something completely new and it
already be in GenBank. Or, if you're lucky, you will have uncovered
new and get famous. Depends on how you look at it! You can PCR from your
semen sample, the fresher the better. The protocols would be similar to
PCRing from whole blood. Good luck!>>>>>>
Thanks for your reply. What is PCR? What is GenBank? I will check at
the hospital and see if they have this but I suspect that they do not. If
necessary, I would PAY someone to do this, as eventually I would like to
publish this case. However, I want the patient to have a "happy ending,"
some relief from the symptoms, and perhaps if I could identify these
organisms therapy would be successful. Would you consider doing it? Or
now someone who would?
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