RCR rumenDNA

[Rohan A VanTwest]

 Dear Netters

  Can someone give me any information on the use of PCR to detect 
Fibrobacter succinogenes and other anaerobic cellulolytic bacteria from the 
bovine rumen.
  The  protocol which I use requires 65 cycles to form a product(500bp) 
that can be  detected by electrophoresis and staining with EtBr. Also the 
primer concentration is a high 200 umol/L compared to the usual 0.05 to 
1.6umol/l.
  The forward primer is  24 bases long and is a rDNA specific sequence 
described by Aman et al 1990 J.Bac.172:762-770.
  The reverse primer is an universal E.coli rDNA sequence.

 We are hope to use this protocol as a lab exercise in our 4th year 
Microbial Ecology course. 

Thanks in advance

Rohan van Twest
Dept of Microbiology
University of Guelph
Guelph Ontario
Canada