Proteases in bacteria: Which?

Doerner Wolfgang doerner at papin.HRZ.Uni-Marburg.DE
Wed Apr 12 07:59:17 EST 1995


Hi Bionetters,

trying to work out a propper protein purification I was just wondering 
which protease inhibitors would be appropriate. Using a trypsine inhi-
bitor for example does not seem to be reasonable to me, since bacteria 
probably don't have those acidic systems. I'm working with cyanobacteria
and prochlorophytes and made a literature research. However, no papers
had apparently been published in the last 10 years, dealing explicitely
with this matter. People usually use a cocktail” of different inhibitors
(if ever), but I would like to know which ones are really needed.
Any help is greatly appreciated,

Wolfgang.

 
   +----------------------------------------------------------------+
   |  Wolfgang Doerner, Fachbereich Biologie/Botanik                |
   |                    Department of Biology/Botany                |
   |                    Karl-von-Frisch Strasse                     |
   |                    Philipps-Universitaet Marburg               |
   |          D - 35032 Marburg, Germany                            |
   |  E-mail: doerner at mailer.uni-marburg.de                         |
   +----------------------------------------------------------------+




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