QUESTION: Library size, coverage

Nathan O. Siemers nathan at
Wed Apr 19 15:14:10 EST 1995


Could someone tell me how you compute the estimated coverage of a
mutant library, given the potential number of members, actual library
size, and the confidence of the result?  For example, say I have made
a library of 3.2 million *possible* members, and have generated 3.5
million clones in the library.  What is the estimated coverage (with
90 or 95% confidence)? 

Any pointers to reference texts would be welcome too :)



Nathan O. Siemers, Ph.D.	
Bristol-Myers Squibb Pharmaceutical Research Institute
3005 First Avenue, Seattle, WA 98121
(206) 727-3741  	siemers at
"You want fries with that?"

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