pH optimum for bacterial growth

Peter Herman herman at populus.slu.se
Fri Aug 11 05:46:42 EST 1995


DR A HARRINGTON, BIOCHEMIE, TEL X482 TYGB (aha at GERGA.SUN.AC.ZA) wrote:
: How does one go about making bacterial culture medium at different ph 
: values? Can the phosphate buffer system be used and at what concentration?
: Are there any pitfalls in the experimental design to avoid? Thanks.

You have to watch the ionic strength of KPhos buffere since some bugs 
have problems with high PO4 directly or because of the effect on Ca

A better choice is often one of the Good buffers like MOPS

Peter
--
************************************************************************
R. Peter Herman				email	Peter.Herman at mv.slu.se
Sveriges Lantbruksuniversitet		Phone:	+46 18 67 12 20
Inst. f. Markvetenskap			Fax:	+46 18 67 27 95
S750 07 Uppsala, Sweden		
************************************************************************



More information about the Microbio mailing list