Spectrophotometric assay for B-lactamases

Poon Wai Mun anomie at singnet.com.sg
Tue Aug 15 18:05:29 EST 1995


Hi ! I'm a student of Ngee Ann Polytechnic. My classmates and I are doing a project on
characterisation of Beta- lactamases.
We need a standard protocol for the spectrometric assay of b-lactamases
DESPERATELY!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
Any kind person who have the protocol, PLEASE reply this note.
Please include the substrate to use, buffer,pH of buffer, amount of substrate and enzyme to add,
the wavelength to scan (lamda maximun) etc.
We shall be eternally grateful to you.
THANK YOU...






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