pH optimum for bacterial growth
Andreas.Brune at uni-konstanz.de
Tue Aug 15 14:02:14 EST 1995
In article <40qg7c$ger at synapse.bms.com>,
mamber at synapse.bms.com (Stephen Mamber) wrote:
>You may be right, but I have had certain situations in performing
>biological assays where sodium and potassium buffers were relatively
>useless. In these situations, I have switched to Good buffers, such as
>Tris and MES, with a noticeable improvement in buffering capacity/
>resistance to adverse pH changes during growth.
Sodium and potassium (ions) are not the buffering species in any case.
And you can hardly avoid them if you make up a MES buffer ;-)
And the buffering capacity is certainly not a parameter governed by the
type of buffer used but its concentration and pK value (relative to the
pH of the medium).
A problem of Good buffers is their price if you want to grow the microorganisms
in large volumes. But I certainly share the reservation against phosphate
buffers. Bicarbonate is indeed a good since natural buffering system.
Unfortunately it calls for a defined (CO2-containing) headspace over the medium.
Dr. Andreas Brune Phone: +49-7531-883282
Mikrobielle Oekologie Fax: +49-7531-882966
Universitaet Konstanz E-mail: Andreas.Brune at uni-konstanz.de
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