AMYLASE mystery--2 attack sites?
brucerat at aol.com
Fri Aug 18 22:10:17 EST 1995
I am a high school teacher using a homemade spectrophotometer to study the
kinetics of salivary amylase digestion of cornstarch. Instead of
following the classic Michaelis-Menten curve, my data indicates that there
may be a secondary mode of action, masked at high substrate concentration,
that becomes apparent as substrate is largely consumed. It shows up as a
slow drift upwards in transmittance. Anyone ever noticed this effect?
Any suggestions? References?
Puzzled, Bruce Ratcliffe (BruceRat at AOL.COM)
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