store_bacillus_4C

Enigl enigl at aol.com
Sat Dec 16 15:37:41 EST 1995


We cultivate the spores on solid plate count agar medium in cake pans at
30-35 C.  Plate count agar seems to work just fine with over 90% spores in
about a week.  Then we scrape off the spores with 70% isopropyl alcohol. 
That kills off most everything except the spores.  Storage in 70% IPA at
2-8 C has been very good with only a one log reduction in ten years.  The
scraped suspension is 10^10 to 10^11 CFU/mL (a thick moist, IPA paste).  




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