Sterilizing with alcohol?
Karl-Heinz Kuhn t2150
kuhn at alf1.ngate.uni-regensburg.de
Wed Dec 20 19:13:12 EST 1995
In article <30d7cb73.0 at news.uni-konstanz.de> Andreas.Brune at uni-konstanz.de (Andreas Brune) writes:
>From: Andreas.Brune at uni-konstanz.de (Andreas Brune)
>Subject: Re: Sterilizing with alcohol?
>Organization: Universitaet Konstanz
>References: <4b7i7l$pna$1 at mhadg.production.compuserve.com>
>X-Newsreader: News Xpress Version 1.0 Beta #4
>Date: Wed, 20 Dec 95 08:38:11 GMT
>Message-ID: <30d7cb73.0 at news.uni-konstanz.de>
>In article <4b7i7l$pna$1 at mhadg.production.compuserve.com>,
> Ben Feldman <103153.3041 at CompuServe.COM> wrote:
>>I need to sterilize some electrodes. If memory serves, a biologist
>>friend once told me that 70% alcohol works well as a disinfectant.
>>Is this true? If so,
>>(1) ethanol or isopropanol?
>>(2) why 70% instead of 100%?
>(1) Both should work, but I never used i-Propanol myself.
Isopropanol is used by people, who need large volumes. Isopropanol is less
expensive than ethanol. That's why!
>(2) 70% EtOH is supposed to be more efficient killing Gram + cells.
> The usual explanation is that 100% EtOH immediately dehydrates the
> murein sacculus which can be very thick with Gram + bacteria
> and then efficiently seals the cytoplasm from contact with the
> alcohol (seemingly logical but to my knowledge not published
> anywhere ...)
>If you are attempting to sterilize electrodes make sure to soak them
>long enough to allow the sterilant to diffuse into any crevices. If you
>are using filling solutions behind a sinter glas diaphragm regard this
>a possible contamination pathway as well.
>Dr. Andreas Brune * Mikrobielle Oekologie * Fakult. f. Biologie * Universitaet
>Konstanz * Postfach 5560, M654 * 78434 Konstanz * Germany * Phone:
>+49-7531-883282 * Fax: +49-7531-882966
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