DNA isolation from Gram-positives?

Marja Tiirola mtiirola at CC.JYU.FI
Fri Dec 29 07:18:01 EST 1995


Hi! Does anybody know methods for cell lysis and 
genomic DNA isolation which are efficient for 
Gram-positive bacteria, too? 

It does not matter although the DNA shears < 10 kb. 
Usual proteinase-K and SDS treatments seem to lyse 
Gram-negatives, but not equally well Gram-positives.
Sonication, bead-mill? Have you experience? 








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