mtiirola at CC.JYU.FI (Marja Tiirola) wrote:
>Hi! Does anybody know methods for cell lysis and
>genomic DNA isolation which are efficient for
>Gram-positive bacteria, too?
>>It does not matter although the DNA shears < 10 kb.
>Usual proteinase-K and SDS treatments seem to lyse
>Gram-negatives, but not equally well Gram-positives.
>Sonication, bead-mill? Have you experience?
>>I use the alkaline/SDS method with an lysozyme pretreament routinely. The DNA does not shear unless it's "manhandled" and is great for Southerns. Qiagen also has columns (G series) and a protocol for genomic DNA isolation. If you need further details, let me know. I'll be back at work Jan.2 and can't get more specifics.