DNA isolation from Gram-positives?

Richard Daines rdaines at q.continuum.net
Fri Dec 29 10:06:16 EST 1995


mtiirola at CC.JYU.FI (Marja Tiirola) wrote:
>Hi! Does anybody know methods for cell lysis and
>genomic DNA isolation which are efficient for
>Gram-positive bacteria, too?
>
>It does not matter although the DNA shears < 10 kb.
>Usual proteinase-K and SDS treatments seem to lyse
>Gram-negatives, but not equally well Gram-positives.
>Sonication, bead-mill? Have you experience?
>
>I use the alkaline/SDS method with an lysozyme pretreament routinely.  The DNA does not shear unless it's "manhandled" and is great for Southerns.  Qiagen also has columns (G series) and a protocol for genomic DNA isolation.  If you need further details, let me know.  I'll be back at work Jan.2 and can't get more specifics.







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