Pseudomonas biofilm composition
RM12 at swt.edu
Sun Feb 5 21:46:09 EST 1995
In response to the query by Tommy on the composition of Pseudomonas biofilms,
there is quite a diverse literature. With regards to the structure of
biofilms, recent articles by Wolfaardt et al. Appl. Environ. Microbiol. 60:
434-446, 1994; and Lawrence et al., J. Bacteriol. 173: 6558 -6567, 1991 employ
confocal scanning laser microscopy and computer imaging to construct three
dimensional images of living, fully hydrated biofilms. Previously, biofilm
architecture had been largely described by electron microscopy. When one
considers that the extracellular capsule material (which is the dominate
feature of biofilms) is ca. 90 - 95% water; many dehydration artifacts arose
during preparation for electron microscopy.
I hope this is helpful.
R.J.C. (Bob) McLean, Ph.D.
Southwest Texas State University
San Marcos, TX 78666
(512)245-2178 dept. for messages
Email: RM12 at swt.edu
More information about the Microbio