synchronous growth of E. coli

Chris=Michiels%LMM%AGR at agr.kuleuven.ac.be Chris=Michiels%LMM%AGR at agr.kuleuven.ac.be
Thu Jan 5 11:36:39 EST 1995


I read in a microbiology textbook that synchronous growth can be the result of
temperature changes, nutrient limitation and other manipulations.
Could someone tell me how synchronization is generally achieved for E. coli,
and how it can be easily monitored?
Is it possible that just diluting an overnight E. coli broth culture grown at 37°C
into fresh medium at 30°C is sufficient for a few synchronized divisions?

Thanks in advance.

Chris Michiels
Lab. Food Microbiology
KU Leuven
Belgium



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