Breaking E.coli cells

EMMERLING,MARCEL GRUEMSCHI at EZINFO.VMSMAIL.ETHZ.CH
Thu Jan 5 15:43:15 EST 1995


Hi,
I want to measure intermediate products of the carbon metabolism of E.coli.
Products like PEP, Pyruvate, Fructose-1,6-bisphosphate, ATP, NAD, NADP etc.
I know how I can detect the isolated intermediate products, but I,m searching
for a method to break the E.coli cells very fast, because I'm interested in
the product concentration at a certain time of growing E.coli strains.

thanx,

	Marcel

gru at biotech.biol.ethz.ch




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