synchronous growth of E. coli

Ives100 ives100 at aol.com
Mon Jan 9 20:33:49 EST 1995


Unfortunately, it is not possible to obtain synchronous growth just by
diluting an 
overnight culture.  The best discussion of bacterial synchronization
techniques, with critiques of each is "Bacterial Growth and Division" by
Stephen Cooper (Academic Press) 1991.  See chapter three for a discussion
and critique of several methods.  Measuring synchrony entails making plate
counts of viable bacteria at frequent (10 min or less) intervals.  Optical
density measurements will not work, as this technique measures cell mass
and not cell number.
                                                                   Tom
Dougherty



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