Que. re. plasmid based bact. genomic library???

taylor patel_p at bms.com
Fri Jan 13 09:45:49 EST 1995


Hi:

	I need to construct a plasmid based bacterial genomic library. 
I have tried the Novogen's "bluestaer vector system" which involves the
cre-mediated excision step and it gave me a very low titer.  I have heard
that there are inherrent problems constructing a genomic library using a
plasmid vector directly.
	I would greatly appreciate any input from anyone has had any 
experience constructing a plasmid based genomic library.  

Thanks in advance

Prem

e-mail:  Patel_P at BMS.com




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