Streptomycin resistant E.coli via rpsL

[Cliff Mintz]

	Based on my experience, rpsL does not confer spectinomycin 
resistant to the strain that carry the mutation.  However, spectinomycin is 
difficult to work with on certain strains of E. coli.  I have also 
encountered low levels of Spr in these strains. Raising the 
concentration as you suggest should do the trick.
			Cliff Mintz
 On Tue, 17 Jan 1995, 
Duncan Clark wrote:

> Hi Folks,
> 
> Does anyone know if an rpsL E.coli ie streptomycin resistant is 
> automatically spectinomycin resistant? I  have the Str/Sp gene 
> from Tn7 on a multicopy plasmid (one of the few antibiotic genes that 
> is stable on v.high copy vectors - the others being Amp and Kan) and 
> use spectinomycin 50ug/ml as my selection. Unfortunately I am finding 
> that my normal strains of choice for other applications are rpsL and 
> give a detectable background on spectinomycin plates. I will take the 
> concn up to 200ug/ml which I know the plasmid quite happily copes 
> with and see if that does the trick. However it would be nice to know 
> if rpsL is automatically Sp resistant.
> 
> Thanks
> 
> Duncan 
> 
> -- 
> -----------------------------------------------------------------------------
> My mind's made up. Don't confuse me with the facts!
> 
> 
>