molybdenum PCR inhibition?

Andre Hamel hamel at cc.umanitoba.ca
Tue Jan 17 18:21:24 EST 1995


Does anybody know concentration of molybdenum that will inhibit PCR?

I've got a danmed feces specimen that contains 10 ppm molybdenum ... DNA 
extracted from this specimen is inhibitory down to 1/100 dilution!

I've tried several sequential DNA extractions, alchohol ppt'ns ... on 
1/2 of my DNA sample, I've also tried competing the Mo with Lithium 
chloride ... no improvement ... likewise with exposure to EGTA and EDTA!

I'm considering chelex.

Any suggestions?  Molbdenum can have +6 charge!

thanks a bunch,

Andre

                            ********************
  Andre Hamel                      email: hamel at cc.umanitoba.ca
  Manitoba Government                         tel: 204/945-7630
  Department of Agriculture                   FAX: 204/945-8062
  Veterinary Services Branch          
  Winnipeg, Manitoba, CANADA 
			  ********************




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