Oxidation Fermentation test

B. Wasilauskas bwasilau at ISNET.IS.WFU.EDU
Thu Jan 19 08:29:32 EST 1995

At 01:32 PM 1/19/95, Sandra Young wrote:
>Would someone be able to provide me with a simple to follow methodology for 
>testing oxidation/fermentation, using Hugh Leifson media. 
>Thank-you in advance 
Inoculate 2 tubes of Hugh Leifson media. Overlay one with sterile mineral 
oil (or strictly speaking you should use sterile petrolatum to insure good 
anaerobic conditions). Incubate for 48 hrs or in some cases it may take up 
to 14 days for reactions to occur. If both tubes turn acidic the organism is 
considered a fermenter, if only the tube without oil turns acidic the 
organism is considered an oxidizer and if no acidity is detected in either 
tube, the organism is considered a nonutilizer of the sugar. Hope this helps.

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