Non-denaturing SDS-PAGE

Guobin Song GSONG at VETMED.TAMU.EDU
Sat Jan 21 14:53:16 EST 1995


Hello! I'm going to run some 35SMet-labeled Brucella
(Gram-negative) whole cell proteins on non-denaturing 
SDS-PAGE.  In order to keep the proteins at non-denature state I can't use SDS and other detergent to lyse the 
cells, I know I can sonicate the cells, but that would be my 
last resort, because I don't want to contaminate our 
sonicator with 35S. So is there any other method availabel 
that can lyse the cells?  Any other suggestions on non-
denaturing SDS-PAGE would be appreciated too.  Thanks!

Guobin Song
Dept. Vet. Pathobiology
TAMU, TX
email: gsong at vetmed.tamu.edu




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