Acid Fast Stains: Gastric Asp.

Jennifer Fuhrman HEQZ65A at prodigy.com
Wed Jul 5 19:50:25 EST 1995


At my hospital (900+ bed), we do acid fast stains on gastric specimens.  
They is no way that you will get false positives.  The organisms that you 
are looking for are bacilli and if you use fluorochrome dyes such as 
auramine and rhodamine with a potassium permanganate counterstain, you 
will not get false positives.   "Normal" bacteria such as Staph aureus do 
not stain and will not stain.  Also, using a partial acid fast stain 
procedure using Carbolfuschin will also not provide false positives 
because the organisms are special and are coated with a thick, waxy 
material that resists staining; however, once stained, the bacteria will 
resist decolorization by strong solvents such as acid alcohol used in the 
fluorochrome stains.  Normal bacteria do not resist staining and will 
over-decolorize...
jen 




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