molecular weight of lps

Gerald Pier gpier at warren.med.harvard.edu
Thu Jul 13 13:57:33 EST 1995


In article <ejames.3.00019692 at cbmse.nrl.navy.mil>,
ejames at cbmse.nrl.navy.mil (Elizabeth. A. James) wrote:

> Does anyone know the molecular weight of the lps of e.coli O128:B12 (monomer) 
> and its chemotype?
> This is going to be one really cool .sig file one day...

Depends on average length of the O side chain.  Since you give an O number
I assume it is not a rough mutant so it wouldn't have a chemotype
analogous to the Ra-Re designations used with Salmonella and E. coli
strains.  Are you working with a rough mutant of this strain?

Also, O antigen length depends on growth conditions, varies from strain to
strain even with the same O type, can decrease during purification if the
glycosidic linkages are somewhat labile, etc.

The molecular weight of lipid A for E. coli is about 1800.  This does not
include any of the core or O side chain sugars.  Can you do something with
this figure?

Jerry Pier
Channing Lab
Harvard Medical School



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