help:purification of mouse antibody IgG2b

r0sesh01 at ulkyvx.louisville.edu r0sesh01 at ulkyvx.louisville.edu
Thu Jul 13 11:48:20 EST 1995


Hi Netters,
	I am working on the purification of monoclonal mouse antibody IgG2b for
my thesis work. I am trying to use hollow fiber modules modified with protein A
or protein G. I am also planning on immobilizing anti-mouse IgG, in the near
future. So far I have been unsuccessful in getting high capacities with protein
A or protein G. 
	I would appreciate if anyone of you could highlight me on any special
conditions/buffers that I might have to use in order to get high capacities.
Please reply to the following e-mail address:
	sjwaig01 at ulkyvm.louisville.edu
Thanks in advance.
								sincerely,
								Sabine.



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