help! - EMS mutagenesis

JLeachCO jleachco at
Mon Jul 17 10:14:57 EST 1995

The key to knowing if you are in the right concentration ranges and time
of treatment is to check "% kill" (i.e. % of the population that doesn't
survive the treatment) and look for a marker which is easy to find (such
as auxotrophy or a drug resistance) to ensure you are really mutating.

Each bug may require some juggling to find the optimum mutation

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