Pseudomonas, Eckhardt and lysis.

[Karine dronen]

I'am gone use Pseudomonas putida in exogenous plasmid isolation.  I want to use the Eckhardt 
procedure fore screening, hoping  to catch the big plasmids.  Unfortunately this Pseudomonas strain 
seems very hard to get lysed with lysozyme, RNase and protease treatment.  So if  there is anyone 
out there who had tried Pseudomonas strains with the Eckhardt technique, please shear your 
experience with me. Also: If there is another successful method for screening Pseudomonas in big 
scale, I would appreciate hearing from you.

Thank you,
Karine Dronen

e-mail address: Karine.Dronen at im.uib.no